Cat. No.
MABL-2554
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
KTM219
From
Recombinant Antibody
Specificity
This antibody bind the C-terminal end of the human osteocalcin.
Alternative Names
BGP; BGLAP; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein
UniProt
P02818
Immunogen
The original antibody was generated by repeatedly immunizing mice with C-terminal hBGP peptide (NH2-FQEAYRRFYGPVCOOH) conjugated with keyhole limpet hemocyanin.
Application Notes
This antibody is recommended for detection of low moecular weight peptides by using open sandwich ELISA (OS-ELISA). This antibody detects C-terminal of human osteocalcin in an ELISA. The cloned VH/VL cDNA fragments of this antibody were successful in detecting C-terminal peptides as short as 6 residues in an OS-ELISA. (PMID: 17636882). The VH-MBP and VL-MBP fusion proteins of this antibody were also used in the detection of small biomarker peptide by a micro OS-ELISA integrated into a microfluidic device (PMID: 20024056). The affinity matured version of this antibody O2AG2 was also made using OS- ELISA. It is also available on request (PMID: 20123883). This antibody was also used in the development of a highly sensitive protein based open sandwich immuno-PCR for detection of small biomarkers (PMID: 24025570)
Antibody First Published
Lim et al. Noncompetitive detection of low molecular weight peptides by open sandwich immunoassay. Anal Chem. (2007); 79(16):6193-200. PMID:17636882
Note on publication
Describes the generation and use of this antibody in the detection of low molecular weight peptides using open sandwich ELISA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
