Cat. No.
MABL-2555
Application
in vitro, in vivo, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
h23C3
From
Recombinant Antibody
Specificity
This antibody is specific for both human and mouse osteopontin.
Alternative Names
BNSP; OPN; SPP-1; PSEC0156; Uropontin; Nephropontin; Bone sialoprotein 1; Urinary stone protein; Secreted phosphoprotein 1
UniProt
P10451
Immunogen
This antibody was raised by immunizing a BALB/c mouse with human osteopontin. Then the antibody was humanized.
Application Notes
The original antibody was used for an ELISA on human osteopontin to determine its specificity. The mouse version of this antibody was used for a western blot on both human and mouse osteopontin, here it was shown that the antibody reacts to both human and mouse osteopontin. This antibody was tested in vivo on CIA mice spleens to test the effect on lymphocytes migration, it showed that the antibody could effectively block lymphocyte migration. Furthermore the antibody could also effectively promote the apoptosis of activated lymphocytes (US20100239588A1, Fan et al., 2008; PMID:18576331). The mouse version of this antibody was used in vitro to determine its effect on ovariectomy-induced osteoporosis, in these experiments it showed that it was able to show significant inhibition in bone loss, further it showed that it was able to reduce mature OC mediated- bone resorption, finally it promotes OC apoptosis (Zhang et al., 2014; PMID:25201732).
Antibody First Published
Fan et al. Treatment of collagen-induced arthritis with an anti-osteopontin monoclonal antibody through promotion of apoptosis of both murine and human activated T cells Arthritis Rheum. 2008 Jul;58(7):2041-52. PMID:18576331
Note on publication
In the original paper they tested the effects of a novel monoclonal antibody (mAb) against human osteopontin (OPN) in the prevention and treatment of collagen-induced arthritis (CIA) and to elucidate the underlying mechanisms of these effects.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
