Cat. No.
MABL-2564
Application
Blocking, FC, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
OX-89
From
Recombinant Antibody
Specificity
This antibody is specific for murine CD252 (the ligand for OX40), expression of which is restricted to activated B cells, dendritic cells, microglia and endothelial cells.
Alternative Names
CD252; Tumor necrosis factor ligand superfamily member 4; OX40 ligand; CD134 ligand; OX40R
UniProt
P43488
Immunogen
This antibody was prepared by immunizing PVG rats with a recombinant chimeric protein consisting of domains 3 and 4 of rat CD4 and the extracellular region of mouse CD134L.
Application Notes
This antibody binds mouse CD134L, and has been shown to bind activated B cells and DCs, as well as CD134L-transfected fibroblast cells, though not activated T cells, in labelling and immunohistochemical analysis (Malmström, 2001). In addition, this antibody is able to partially block the interaction between CD134 and CD134L. This blocking effect could be responsible for the ability of this antibody to prevent T cell accumulation in the intestine of T cell-restored SCID mice and inhibits the development of Th1-mediated colitis.
Antibody First Published
Malmström et al. CD134L Expression on Dendritic Cells in the Mesenteric Lymph Nodes Drives Colitis in T Cell-Restored SCID Mice J Immunol. 2001 Jun 1;166(11):6972-81. PMID:11359859
Note on publication
Describes the original use of this antibody in blocking studies to implicate CD134L expression on DCs in mouse mesenteric lymph nodes in the development of colitis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
