Cat. No.
MABL-2565
Application
functional assay, RIA, WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rabbit, Human, Mouse
Clone No.
E06
From
Recombinant Antibody
Specificity
This antibody is specific for oxidized lipoproteins.
Alternative Names
OxPL; OxLDL; CuOx-LDL; copper-oxidized low-density lipoproteins; EO6
UniProt
Immunogen
The original mouse antibody was raised from apoE-deficient mice without any external immunogens. They were fed a high fat diet containing 12.8% milk fat and 0.15% cholesterol for 7 months. Such dietary intervention induces extensive aortic atherosclerosis, and is accompanied by the formation of high titers of E0 antibodies to MDA-lysine.
Application Notes
To determine the antibody's binding affinity to native or modified human LDL, a radioimmunoassay was conducted using the mouse version of this antibody. Immunocytochemistry was performed on atherosclerotic lesions of LDL receptor-deficient rabbits, balloon-catheterized, cholesterol-fed New Zealand white rabbits, and human brain arteries using the mouse version of this antibody (Palinski et al., 1996; PMID: 8698873). The mouse version of this antibody was characterized through various experiments and assays. An antibody absorption experiment was conducted on increasing concentrations of native PAPC, OxPAPC, or microemulsions made from lipids extracted from native LDL or CuOx-LDL. Additionally, it was used in an ELISA performed on antigens in PBS. In a Western blot assay, the alkaline phosphatase-labeled goat anti-mouse IgM version of this antibody was utilized to detect CuOx-LDL and native LDL. A flow cytometric experiment was performed on resident peritoneal macrophages isolated from female Swiss-Webster mice using the original mouse version of this antibody. Furthermore, a binding and degradation assay was carried out on mouse peritoneal macrophages using the mouse version of this antibody (Hörkkö et al., 1999; PMID: 9884341).
Antibody First Published
Palinski et al. Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma J Clin Invest. 1996 Aug 1;98(3):800-14. PMID:8698873
Note on publication
The original publication discusses the cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice ("E0 antibodies"), demonstrating the occurrence of these epitopes in human plasma and atherosclerotic lesions, and highlighting the immunological response to various epitopes of oxidized lipoproteins in vivo.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
