Cat. No.
MABL-2568
Application
IP, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
6A9
From
Recombinant Antibody
Specificity
The antibody is specific for P-Cadherin-1 (CDH3). P-Cadherin-1 is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail.
Alternative Names
Cadherin 3 type 1; Cadherin-3; Cadp; Calcium dependent adhesion protein placental; CDH3; CDHP; HJMD; P-cadherin (Placental); PCAD; Placental cadherin
UniProt
P22223
Immunogen
The original antibody was generated by immunizing mice against a 72-kilodalton (kDa) P- cadherin fragment from A-431 cells.
Application Notes
The antibody detected human P-Cadherin from A431 cell lysate by Western blot analysis. The antibody was used to immunoprecipitate P-Cadherin. Further, the antibody was shown to have inhibitory activity, by disrupting P-cadherin-mediated cell-cell adhesion with A-431 cells (Lewis et al., 1994; PMID: 8006450). The antibody detected P-cadherin by immunofluorescence (Owens et al., 2000; PMID: 10708559).
Antibody First Published
Lewis et al. Cadherin function is required for human keratinocytes to assemble desmosomes and stratify in response to calcium J Invest Dermatol. 1994 Jun;102(6):870-7. PMID:8006450
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
