Cat. No.
MABL-2582
Application
IP, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Xenopus, Human, Mouse
Clone No.
X77
From
Recombinant Antibody
Specificity
This antibody recognizes the human p73 protein as well as the human and xenopus p53 protein.
Alternative Names
Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
UniProt
P04637
Immunogen
The original mouse IgG1 version of this antibody was raised by immunizing mice against xenopus p53
Application Notes
This original mouse IgG1 can be used to detect human p73 as well as human and xenopus p53. The reaction of this antibody with the two proteins was verified using immunoprecipitation and western blot (Le Bras et al., 2002; PMID: 11850851). The same group used indirect immunofluorescence to verify the specificity of the antibody (Le Bras et al., 2002; PMID: 11850851). Another group used the antibody with immunoprecipitation and immunoblotting to detect the accumulation of human p53 with increasing levels of calpastatin (Pariat et al., 1997; PMID: 9111352).
Antibody First Published
Le Bras et al. Monoclonal antibodies raised against Xenopus p53 interact with human p73 Oncogene. 2002 Feb 14;21(8):1304-8 PMID:11850851
Note on publication
Describes the characterization of the original X77 clone which was generated in the same paper
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
