Cat. No.
MABL-2586
Application
WB, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
S11E
From
Recombinant Antibody
Specificity
This antibody is specific for the first coiled-coil region of the microtubule-associated protein p150glued.
Alternative Names
p150glued; p150-glued; DAP-150; DP-150; p135; Dynactin subunit 1; 150 kDa dynein-associated polypeptide
UniProt
Immunogen
The original antibody was generated via phage display from the Griffin.1 library by screening against Microtubule-Binding Proteins (MBPs) derived from HeLa S3 cells.
Application Notes
The original format of this antibody (scFv) was successfully used in a Western blot and for in vitro immunofluorescence staining of p150glued in HeLa cells (Nizak et al., 2003; PMID: 14617357).
Antibody First Published
Nizak et al. Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo Traffic. 2003 Nov;4(11):739-53. doi: 10.1034/j.1600- 0854.2003.00132.x PMID:14617357
Note on publication
The original publication demonstrates the use of recombinant antibodies selected through phage display against subcellular fractions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
