Cat. No.
MABL-2585
Application
FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BU5
From
Recombinant Antibody
Specificity
This antibody is specific for human p63, a type II transmembrane protein identified in the rough endoplasmic reticulum. Surface expression of p63 is characteristic of differentiated plasma cells, due to their high secretory activity. As a result, p63 acts as a plasma cell marker. It also plays an important role in the development of epithelial tissues.
Alternative Names
Tumor protein 63; Chronic ulcerative stomatitis protein; CUSP; Keratinocyte transcription factor; KET; Transformation-related protein 63; TP63; Tumor protein p73-like; p73L; p40; p51
UniProt
Q9H3D4
Immunogen
This antibody was raised by immunising mice with cells of the human myeloma cell line RPMI- 8226. Spleen cells from immunised mice were fused to cells of a BALB/c plasmacytoma cell line.
Application Notes
This antibody has been used to detect cells expressing p63, which is characteristic of B cells, in flow cytometric analysis of B cell surface antigen expression (Exley et al, 1987), and, similarly, to assess the extent of differentiation of Daudi cells towards the plasma cell phenotype by flow cytometry (Clemens et al, 1988). In immunofluorescence assays, this antibody strongly stains plasma cells in acetone- fixed human tonsil sections, particularly in inter-follicular T-dependent areas, in and around germinal centres and in subepithelial areas (Johnson et al, 1985). This antibody also strongly stains acinar epithelial cells in thyroid sections.
Antibody First Published
Johnson et al Use of monoclonal antibodies reactive with secretory epithelial cells for the immunocytochemical identification of plasma cells. Immunol Lett. 1986 Jun;12(5-6):295-300. PMID:3733159
Note on publication
Describes the original generation and characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
