Cat. No.
MABL-2584
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
60738-10D8
From
Recombinant Antibody
Specificity
This antibody binds human p63. It acts as a sequence specific DNA binding transcriptional activator or repressor. It is also involved in notch signaling by probably inducing JAG1 and JAG2 and plays a role in the regulation of epithelial morphogenesis. The p63 molecule has a high degree of structural homology with p53 and has three similar functional domains: N-terminal transcription activation region, core DNA binding region, and C-terminal oligomerization region.
Alternative Names
TP63; CUSP; Tumor protein 63; chronic ulcerative stomatitis protein; Keratinocyte transcription factor KET; Transformation-related protein 63; Tumor protein p73-like; p73L; p40; p51; 4A4
UniProt
Q9H3D4
Immunogen
The original antibody was generated by immunizing 4-6 weeks old female BALB/c mice with recombinant human p63 protein having a histidine tag.
Application Notes
This antibody is highly similar to commercially available antibody 4A4 in immunohistochemical staining of p63. This antibody was used for the immunohistochemical staining of prostate cancer and breast cancer tissues. This binding specificity and affinity of this antibody was determined using ELISA. The original IgG1 antibody is reported to bind human p63 with an affinity constant of 2.47 X 10^9 M^(-1). Purified 60738-10D8 antibody can specifically detect p63 recombinant protein in western blot. (CN104447994)
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
