Cat. No.
MABL-2591
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Clone No.
3A3
From
Recombinant Antibody
Specificity
This antibody is specific for paclitaxel, an anti-neoplastic agent derived from the Pacific yew. It displays low cross-reactivity with other naturally-occurring taxanes.
Alternative Names
PT; PTX; Taxol; 7-Xylosylpaclitaxel; Taxol-7-xyloside; 7-xylosyltaxol
UniProt
Immunogen
This antibody was raised by immunising BALB/c mice with a solution of 7-xylosyltaxol conjugated to the carrier protein bovine serum albumin (XylTax–BSA).7-xylosyltaxol is a taxol (paclitaxel) derivative, which binds to tubulin and inhibits microtubule dis
Application Notes
This antibody has been used in an indirect competitive enzyme-linked immunosorbent assay to determine the paclitaxel content of yew tree samples, with a detection range of 0.098–312.5 μg ml−1 (Chao et al, 2012). An scFv derived from this antibody reacts with both free and immobilised paclitaxel in icELISA analysis, and exhibits less cross-reactivity for paclitaxel-related compounds (Yusakul et al, 2016).
Antibody First Published
Chao et al. Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against paclitaxel J Nat Med (2013) Volume 67, Issue 3, pp 512–518 PMID:23007175
Note on publication
Describes the original generation of this antibody, and its use in an indirect competitive enzyme-linked immunosorbent assay.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
