Cat. No.
MABL-2594
Application
inhibit, neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Pig, Human
Clone No.
8H9D4
From
Recombinant Antibody
Specificity
This antibody binds human PAI-1 at an epitope between amino acids 277 and 327, more particularly it binds residues between Arg300, Gln303 and Asp305 located on the loop between α-helix I and β-strand 5A. This antibody cross-reacts with pig PAI-1 but not with mouse or rat. Plasminogen activator inhibitor-1 (PAI-1), is a serine protease inhibitor, a unique member of the serpin superfamily which plays an important role in fibrinolysis and is an established risk factor for cardiovascular diseases. It a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading .
Alternative Names
SERPINE1; PAI1; PAI; PLANH1; Plasminogen activator inhibitor 1; Endothelial plasminogen activator inhibitor; Serpin E1; MA-8H9D4; APG
UniProt
P05121
Immunogen
The original antibody was generated using PAI-1 complex as an immunogen.
Application Notes
This antibody is reported to have strong inhibitory effect towards PAI-1 activity. The cross reactivity of this antibody towards other PAI-1 than human was done using ELISA (PMID: 9048903). This antibody shifts the reaction to the substrate pathway without decreasing the rate-limiting constant for reactive center loop insertion by tPA (PMID: 12223472). This antibody interferes with the final locking step in the serpin/proteinase interaction, thereby explaining its substrate inducing properties (PMID: 12871373).
Antibody First Published
Debrock et al. Neutralization of plasminogen activator inhibitor-1 inhibitory properties: identification of two different mechanisms. Biochim Biophys Acta. 1997 Feb 8;1337(2):257-66. PMID:9048903
Note on publication
The paper investigates two mechanisms of neutralization of the inhibitory activity of PAI-1 using monoclonal antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
