Cat. No.
MABL-2595
Application
inhibit, neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
MEDI-579
From
Recombinant Antibody
Specificity
This antibody binds the reactive center loop (RCL) of human PAI-1 and at the same exosite used by both tissue and urokinase plasminogen activators (tPA and uPA). This antibody binds the active form of human PAI-1. Plasminogen activator inhibitor-1 (PAI-1), is a serine protease inhibitor, a unique member of the serpin superfamily which plays an important role in fibrinolysis and is an established risk factor for cardiovascular diseases. It a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading .
Alternative Names
SERPINE1; PAI1; PAI; PLANH1; Plasminogen activator inhibitor 1; Endothelial plasminogen activator inhibitor; Serpin E1; Humanized Ab167; MEDI579; Antibody 08
UniProt
P05121
Immunogen
The original antibody was isolated from a human scFv library by panning on recombinant human and rat PAI-1 followed by screening in a PAI-1/tPA competition binding assay.
Application Notes
This antibody specifically inhibits serine protease interactions with PAI-1 while conserving vitronectin binding. The binding of this antibody to native and latent form of human PAI-1 was determined using ELISA. This antibody was able to neutralize recombinant human, rat and mouse PAI-1 activity in species-relevant tPA-coupled chromogenic assays. The Fab version of this antibody binds human and rat PAI-1 with a binding affinity (Kd) of 5.34 pM and 84 pM respectively. Competition binding experiments also demonstrates that MEDI-579 does not interact with other circulating serpins (PMID: 30733557). This antibody is effective in slowing the progression of diabetic nephropathy in db/db mice and that the effect is additive to ACEI (PMID: 27511457).
Antibody First Published
Vousden et al. Discovery and characterization of an antibody that selectively modulates the inhibitory activity of plasminogen activator inhibitor-1. Sci Rep. 2019 Feb 7;9(1):1605. PMID:30733557
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
