Cat. No.
MABL-2596
Application
functional assay, inhibit, neutralize
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Nb42 (VHH-2g-42)
From
Recombinant Antibody
Specificity
This antibody binds to a conformational epitope on human PAI-1 which comprises the loop connecting strands 1 and 2 of β-sheet B (s1B and s2B) as well as the proximal portions of each strand. Plasminogen activator inhibitor-1 (PAI-1), is a serine protease inhibitor, a unique member of the serpin superfamily which plays an important role in fibrinolysis and is an established risk factor for cardiovascular diseases. It a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading .
Alternative Names
SERPINE1; PAI1; PAI; PLANH1; Plasminogen activator inhibitor 1; Endothelial plasminogen activator inhibitor; Serpin E1
UniProt
P05121
Immunogen
The original antibody was isolated from a nanobody library generated by immunization of three alpacas with three different PAI-1 variants.
Application Notes
This antibody is reported to exhibit moderate dose-dependent PAI-1 inhibition. This antibody is capable of inhibiting PAI-1 activity by directly preventing the initial PAI-1.t-PA complex formation (PMID: 27604413). This antibody interferes with exosite interactions between 37-loop of proteinase and negatively charges residues of the RCL and affects the formation of Michaelis complex and dissociation of acyl enzyme (Komissarov et al, 2019).
Antibody First Published
Zhou et al. Generation and in vitro characterization of inhibitory nanobodies towards plasminogen activator inhibitor 1. Thromb Haemost. 2016 Nov 30;116(6):1032-1040. PMID:27604413
Note on publication
Describes the generation of 5 inhibitory nanobodies against PAI-1.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
