Cat. No.
MABL-2609
Application
neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HuMBC1
From
Recombinant Antibody
Specificity
HuMBC1 binds to human PTHrPat an epitope between residues 20 and 30(KD ~ 0.2 nM), a site closely overlapping with the GPCR parathyroid hormone receptor 1 (PTH1R) binding site. The antibody does not bind to PTH at concentrations of 10ug/ml. PTHrP is known to be a causative factor of humoural hypercalcemia of malignancy (HHM), which is frequently found at advanced stages of cancer. The protein binds to PTHR1, which is expressed on the surface of cells of the osteoblast lineage and of the kidney tubule; this interaction actively promotes bone resorption, in a manner identical to that of PTH.
Alternative Names
hu-q
UniProt
Immunogen
The anti-PTHrP antibody was prepared by immunizing mice with synthetic bovine thyroglobulin-conjugated PTHrP (aa 1-34) and generating the hybridoma cell line #23-57-137-1. The CRD regions of the variable region of the heavy chain was grafted onto the human framework regions of the human antibody S31679, and for the light chain FR1, FR2 and FR3 were grafted to that of HSU03868 and FR4 of the mouse antibody was grafted to that of S31679.
Application Notes
HuMBC1 can almost completely blocks the PTHrP-induced cAMP production in rat osteosarcoma cells that expressed PTHR1, even in the presence of 2 g/mL PTHrP(1-34) – the neutralizing activity in the humanized antibody is equilavent to that of the murine antibody from which it is derived (#23-57-137-1). The humanized antibody is also effective in reducing the blood ionized calcium levels of hypercalcemic mice bearing the PAN-7-JCK human pancreatic cancer xenograft or the LC-6-JCK human lung cancer xenograft that secreted PTHrP. PTH binding and activity is not neutralized by this antibody. The antibody can also be used to detect the presence of and binding of PTHrP by EILSA.
Antibody First Published
Onuma et al. Generation of a Humanized Monoclonal Antibody Against Human Parathyroid Hormone-related Protein and its Efficacy Against Humoral Hypercalcemia of Malignancy Anticancer Res. 2004 Sep-Oct;24(5A):2665-73. PMID:15517871
Note on publication
Describes the generation of a humanized anti-hPTHrP mAb. Its effects in vivo and in vitro were studied.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
