Cat. No.
MABL-2627
Application
inhibit, therapeutic, WB, Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
PA4
From
Recombinant Antibody
Specificity
This antibody binds human PCSK9. Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a serine protease enzyme encoded by the PCSK9 gene in humans and it plays a role in regulation of circulating cholesterol. It is associated with autosomal dominant hypercholesterolemia, a state of elevated levels of LDL (low-density lipoprotein) cholesterol. Autosomal dominant hypercholesterolemia can result in severe implications such as stroke and coronary heart disease.
Alternative Names
NARC1; NARC-1; PC9; Proprotein convertase subtilisin/kexin type; Neural apoptosis-regulated convertase 1; Proprotein convertase 9; Subtilisin/kexin-like protease PC9; PA4Fab
UniProt
Q8NBP7
Immunogen
The original antibody was isolated from a natural human Fab phage display library by panning against recombinant human PCSK9. Later one the Fab antibody was converted to IgG1 format.
Application Notes
The human IgG1 format of this antibody was reported to bind human PCSK9 with a binding affinity Kd= 1.05 nM. The binding characterization of this antibody to human PCSK9 was done using ELISA and western blot. In an in vitro competition binding ELISA, IgG1-PA4 blocked PCSK9 from binding to LDLR, with an IC50 of approximately 7 nM. In vitro, it also attenuated PCSK9-mediated degradation of LDLR on the HepG2 cell surface in a dose dependent manner. In C57BL/6 mice, administration of IgG1-PA4 at 30 mg/kg increased hepatic LDLR protein levels by as much as 3 fold when compared with control. This antibody has potential for being used as a therapeutic for the treatment of hypercholesterolemia by inhibiting PCSK9-mediated degradation of cell surface LDLRs (PMID: 26056005).
Antibody First Published
Cao et al. Selection and characterization of human PCSK9 antibody from phage displayed antibody library. Biochem Biophys Res Commun. 2015 Aug 7;463(4):712-8. PMID:26056005
Note on publication
This paper describes the isolation of a Fab antibody that binds human PCSK9. The IgG1 format was generated and characterized.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
