Cat. No.
MABL-2632
Application
therapeutic, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Rhesus Monkey
Clone No.
1G4
From
Recombinant Antibody
Specificity
This antibody binds human PD-1 protein. PD-1 acts as an inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. It delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2.
Alternative Names
CD279; PD1; PD 1; hPD-1; PDCD1; Programmed cell death protein 1; Programmed cell death 1; Protein PD-1; 41-1G4; 1G4-mHvKv
UniProt
Q15116
Immunogen
The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with his-tagged human PD-1 protein.
Application Notes
The initial binding characterization of the antibody was done using ELISA. The binding reactivity of this antibody to human, mouse and rhesus monkey PD-1 expressed on CHO cells was determined using flow cytometry. This antibody was shown to cross react with human and rhesus monkey PD-1 and not mouse PD-1. The binding affinity of the original mouse IgG4 antibody for human PD-1 was determined using surface plasmon resonance and was found to be Kd= 1.07 nM. The anti-tumor effect of this antibody was tested in vivo in a mouse colon cancer model. Murine MC-38 cells (colon adenocarcinoma cells) were injected subcutaneously in B-hPD-1 mice and treated with this antibody. It was reported that this antibody showed good tumor inhibitory effect (CN111763257).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.