Cat. No.
MABL-2640
Application
FC, WB, IHC, ELISA
Isotype
Engineer antibody
Species Reactivity
Dog
Clone No.
JC053
From
Recombinant Antibody
Specificity
This antibody binds canine PD-1.
Alternative Names
CD279; Programmed death 1 receptor; PD1; PD 1
UniProt
A0A024FCJ9
Immunogen
The original antibody was generated by immunizing BALB/c mice with recombinant canine PD-1 using an exponentially increasing dosing strategy with MPLA as adjuvant.
Application Notes
This antibody is recommended for detection of canine PD-1. This antibody can detect canine PD-1 in a western blot assay. JC053 successfully detected canine PD-1 expressed on formalin-fixed paraffin-embedded (FFPE) tonsil and lymph node via immunohistochemistry suggesting that this could be useful for histological detection of canine PD-1 and as diagnostic tool in tumor biopsies. JC053 was also used to determine the PD-1 expression on CD4+ and CD8+ T-cells of canine PBMCs stimulated with ConA using flow cytometry. It could also detect recombinant canine PD-1 expression on CHO and CLGL-90 cells in flow cytometry. The ability of the antibody to block interactions between PD-1 and PD-L1 was checked using ELISA. It is known that blockade of PD-L1 and PD-1 interactions causes increase in Ifn-γ level. This antibody did not effectively block PD-1 and did not cause a recovery of T cell function (PMID: 32614928).
Antibody First Published
Choi et al. Development of canine PD-1/PD-L1 specific monoclonal antibodies and amplification of canine T cell function. PLoS One. (2020); 15(7): e0235518 PMID:32614928
Note on publication
Describes the generation and characterization of antibodies towards canine PD-1 and PD-L1 for their potential use in therapeutics for veterinary oncology.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
