Cat. No.
MABL-2653
Application
Blocking, in vitro, in vivo, inhibition, SPR
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
BT613
From
Recombinant Antibody
Specificity
The original antibody is specific for PD-L1. The antibody recognises human and mouse PD-L1.
Alternative Names
CD274; B7-H1; B7 homolog 1; PDCD1 ligand 1; Programmed death ligand 1; hPD-L1; Programmed cell death 1 ligand 1
UniProt
Q9NZQ7
Immunogen
The original antibody was generated by immunizing transgenic mice with PD-L1, followed by antibody library construction and phage display-based selection against PD-L1.
Application Notes
The binding affinity of the antibody to PD-L1 was measured by surface plasmon resonance. The binding affinity of the antibody was measured on the cell lines with high expression of PD-L1 by FACS. The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody had good activity in blocking the binding of PD-L1 to PD-1 and PD-L1 to B7-1 is shown by ELISA analysis. The antibody could block the binding of PD-1 and PD-L1 at the cellular level in in vitro experiments. The antibody was used for in vivo experiments on colon cancer tumor model. B-hPD-1 mice with human PD- 1 and MC38-hPD-L1 colon cancer cells with human PD-L1 were treated with the antibody. Results showed inhibition of tumor volume growth in mice treated with the antibody.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
