Cat. No.
MABL-2656
Application
FC, WB, ELISA, Block
Isotype
Engineer antibody
Species Reactivity
Dog
Clone No.
JC071
From
Recombinant Antibody
Specificity
This antibody binds canine PD-L1.
Alternative Names
CD274; Programmed death ligand 1; PDL-1; PDL1
UniProt
E2RKZ5
Immunogen
The original antibody was generated by immunizing BALB/c mice with recombinant canine PD-L1 using an exponentially increasing dosing strategy with MPLA as adjuvant.
Application Notes
This antibody is recommended for detection of canine PD-L1. This antibody successfully detected canine PD-L1 in a western blot assay. JC071 was used to determine the increased PD- 1 expression on canine PBMCs stimulated with peptidoglycan or lipopolysaccharide using flow cytometry. JC071 was also able to detect recombinant PD-L1 expressed on DH82 cell line both with and without in vitro activation using IFN-γ in flow cytometry. It also recognized recombinant canine PD-L1 expressed on CHO cells in flow cytometry. The ability of the antibody to block interactions between PD-1 and PD-L1 was checked using ELISA. It is known that blockade of PD-L1 and PD-1 interactions causes increase in Ifn-γ level. JC071 successfully blocked PD-L1 and amplified IFN-γ production in culture by approximately 6-fold. Thus, JC071 alleviated T-cell suppression and led to enhanced functional activity of T cells in culture and have potential application as an immunotherapy reagent for cancer treatment (PMID: 32614928).
Antibody First Published
Choi et al. Development of canine PD-1/PD-L1 specific monoclonal antibodies and amplification of canine T cell function. PLoS One. (2020); 15(7): e0235518 PMID:32614928
Note on publication
Describes the generation and characterization of antibodies towards canine PD-1 and PD-L1 for their potential use in therapeutics for veterinary oncology.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
