Cat. No.
MABL-2655
Application
Blocking, in vivo, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
D7A8
From
Recombinant Antibody
Specificity
The antibody is specific for PD-L1.
Alternative Names
CD274; B7-H1; hPD-L1; PDCD1 ligand 1; Programmed cell death 1 ligand 1; B7 homolog 1
UniProt
Q9NZQ7
Immunogen
The original antibody was isolated by panning a phage display library against PD-L1.
Application Notes
The scFv format of the antibody,together with B1C4A7, was used for constructing in the bispecific antibody (A7-A8)-LALA. The scFv format and the bispecific antibody could strongly bind to the recombinant human and mouse PDL1 (EC50 = 0.121 nM and 0.172 nM, respectively for scFv and 0.088 nM and 0.160 nM, respectively for the bispecific antibody). The scFv format and the bispecific antibody could strongly block ligand PDL1 binding to its receptor PD1. IC50 for hPDL1-hPD1 interaction was 2.51 nM for the bispecific antibody, and 3.04 nM for dsD7A8-LALA. IC50 for the mPDL1-mPD1 interaction was 5.59 nM for the bispecific antibody, versus 2.80 nM for dsD7A8-LALA. The bispecific antibody could bind to cell expressed human PDL1 and mouse PDL1. The EC50 values of the bispecific antibody to MDA-MB-231 and B16-F10 were 0.223 nM and 0.122 nM, respectively; for dsD7A8 to MDA-MB-231 and B16-F10 the values were 0.159 nM and 0.042 nM, respectively.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
