Cat. No.
MABL-2667
Application
Block, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HZ-D-Ye-29-3
From
Recombinant Antibody
Specificity
This antibody binds human PD-L2, expressed on dendritic cells, IFN-gamma-treated monocytes, placental endothelium and medullary thymic epithelial cells. This protein is an immune checkpoint receptor ligand which plays a role in negative regulation of adaptive immune response. It is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner.
Alternative Names
CD273; PD-L2; PD L2; PDCD1LG2; PDCD1L2; B7DC; B7-DC; PD-1 ligand 2; PDCD1 ligand 2; PDCD1 ligand 2; Programmed cell death 1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC
UniProt
Q9BQ51
Immunogen
The original antibody was generated by immunizing 2 alpacas with human PDL2 antigen in Freund's adjuvant. The peripheral blood of the alpaca was taken to generate a yeast display nanobody library and the particular clone was isolated by screening the library against human PDL2.
Application Notes
This antibody binds human PD-L2 with a binding affinity of Kd= 4.49 nM. The binding ability of this antibody towards human PD-L2 expressed on the surface of CHO cells was determined using flow cytometry. This antibody is capable of blocking the interaction between PDL2 and PD-1. This antibody in combination with an anti-PDL1 antibody 14-02-3 was used in the generation of a bispecific antibody targeting human PDL1 and PDL2 simultaneously. The resulting bispecific antibody was capable of blocking binding of PD-L2/PD-L1 to PD-1 and also block PDL1/PDL2/PD1/luc signaling pathway (WO2021197358).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
