Cat. No.
MABL-2676
Application
crystallization, SPR, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Plasmodium falciparum
Clone No.
B9
From
Recombinant Antibody
Specificity
The antibody recognizes Pf12p. The antibody does not cross-react with Pf12 or Pf41. The antibody binds specifically to the Pf12p interdomain (D1-D2 junction) area.
Alternative Names
Surface protein P12p
UniProt
C6KSX1
Immunogen
The original antibody was generated by immunizing an alpaca with recombinant Pf12p protein and constructing a phage library. The antibody was isolated by biopanning against Pf12p.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody recognised reduced and non-reduced Pf12p in western blot analysis. The binding affinity of the original format of the antibody to Pf12p was measured by surface plasmon resonance (Kd= 6.7 nM). The structure of the antibody in complex with recombinant Pf12p was determined by X-ray crystallography (Dietrich et al., 2021; PMID: 33480416).
Antibody First Published
Dietrich et al. Nanobody generation and structuralcharacterization of Plasmodium falciparum6-cysteine protein Pf12p Biochem J. 2021 Feb 12;478(3):579-595. doi: 10.1042/BCJ20200415. PMID:33480416
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
