Cat. No.
MABL-2682
Application
IF;
Isotype
Engineer antibody
Species Reactivity
Caenorhabditis elegans
Clone No.
KT22
From
Recombinant Antibody
Specificity
This antibody recognized the apical side of the pharynx of Caenorhabditis elegans. Caenorhabditis elegans is a transparent nematode that lives in temperate soil environments.
Alternative Names
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with subtracted embryonic extract. Subtracted extracts were prepared by using a method called “antigen subtraction” which enriched antigens of low abundance in heterogeneous molecule mixtures by subtracting abundant antigens.
Application Notes
This antibody was used for the staining of the apical region of the pharynx of Caenorhabditis elegans. The KT22 signal was detectable in the twofold embryo as a single line from the buccal cavity to the end of isthmus; at the pretzel stage, the KT22 stained three lines from the buccal cavity to the anterior isthmus, which appeared to merge into a single line in the posterior isthmus region. No KT22 signal was detected in the terminal bulb and pharyngeal–intestinal valve (PMID; 18498356).
Antibody First Published
Takeda et al. Efficient production of monoclonal antibodies recognizing specific structures in Caenorhabditis elegans embryos using an antigen subtraction method. Genes Cells. 2008 Jul;13(7):653-65. PMID:18498356
Note on publication
Describes the use of "antigen subtraction" method to enrich antigens of low abundance in heterogeneous molecule mixtures by subtracting abundant antigens. The paper also discloses generation of 35 mAbs against Caenorhabditis elegans that recognize specific structures, including P granules, muscles, the pharynx, and subsets of hypodermal cells; some of the mAbs revealed previously unreported cellular structures.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
