Cat. No.
MABL-2699
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Naja nigricollis
Clone No.
TPL004_01_A11
From
Recombinant Antibody
Specificity
TPL004_01_A11 antibody recognizes PLA2 (Phospholipase A2 'basic'), which is one of the major proteins in the venom of the black-necked spitting cobra (Naja nigricollis).
Alternative Names
Phospholipase A2 'basic'; Phospholipase A2; svPLA2; Phosphatidylcholine 2-acylhydrolase; Phospholipase A2 isozyme III/IV; CM-III/CM-IV
UniProt
P00605
Immunogen
The antibody was raised using IONTAS phage display library, a naïve human scFv library consisting of 4.0 · 10^10 unique antibody fragments. Through phage display selection, scFv-presenting phages were allowed to bind the venom proteins, phages with unspecific binding were washed away, and the rest were eluted through enzymatic digestion. These were then amplified and used for the following round. In total three rounds of selections were completed leaving the best binders behind.
Application Notes
TPL004_01_A11 antibody has a very high affinity to the PLA2. It was demonstrated by normalized ELISA, which circumvents the limitation of a simple ELISA by removing the expression variable, normalizing the amount of scFv in each well. The antibody's high affinity was confirmed by its ability to bind the target protein in a venom fraction even at 1:32,000 dilution (3.125 · 10^-2) µg/mL (Jürgensen et al., 2019 [poster]). It is recommended for the research on the antitoxin against Naja nigricollis snakebites.
Antibody First Published
Jürgensen et al. Harnessing monoclonal antibodies for development of a specific treatment against Naja nigricollis envenoming. 10.13140/RG.2.2.24771.14884.
Note on publication
This poster describes the generation and characterization of the TPL004_01_A11 antibody among other antibodies directed against the venom toxins of Naja nigricollis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
