Cat. No.
MABL-2705
Application
IP, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
AbAb1-PLCy
From
Recombinant Antibody
Specificity
This antibody binds phosphorylated phosphoinositide phospholipase C-gamma, which mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). It also plays an important role in the regulation of intracellular signaling cascades.
Alternative Names
PLCG1; PLC1; PLC-gamma-1; PLC-γ; 1-phosphatidylinositol 4,5- bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1
UniProt
P19174
Immunogen
The original antibody was generated by immunizing rabbits with recombinant phosphorylated peptide pPLC-γ(RNPGF-pY-VEANPGGGSC) conjugated with KLH as an immunogen.
Application Notes
The binding reactivity of this antibody to recombinant phosphorylated pPLC-γ peptide was confirmed using ELISA. The binding reactivity was further confirmed using western blot, of phosphorylated PLC-γ from NIH3T3 cell lysates. The scFv version of this antibody was used fused to human IgG1 using a suitable linker for the generation of scFv-Fc mini-antibody. This minibody was capable of immunoprecipitating pPLC-γ. The fluorescent scFv version of this antibody, when expressed intracellularly was reported to inhibit signal transduction by PLC-γ in transfected into NIH3T3 cells as observed using fluorescent microscopy (US20090233358).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
