Cat. No.
MABL-2714
Application
IP, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1C2
From
Recombinant Antibody
Specificity
This antibody bind the extended polyglutamine repeats found in the TATA binding protein. It is reported that the affinity of this mAb is proportional to the length of the polyglutamine chain and that this antibody recognizes polyQ stretches with 11, 22 and 41 amino acids.
Alternative Names
PolyQ; Glutamine repeats; TATA binding protein; TBP
UniProt
P20226
Immunogen
This antibody was raised against the full length TATA binding protein (TBP) containing a poly Q sequnce with 38 repeats of glutamine and collected from the ascites fluid of the mice.
Application Notes
This antibody recognizes recombinant and endogenous HeLa cell TBP in Western blots. Epitope mapping of the antibody was done using ELISA by incubating the mAbs with synthetic overlapping peptides having 150 amino acids from the N-terminal of the HeLa cell TBP. Thia antibody immunoprecipitates natibe TFIID from phosphocellulose 1 M KCl fractions indicating that the N-terminal region of TBP is not blocked by TBP binding proteins. It is reported that mAb 1C2 selectively inhibited in vitro transcription from TATA-containing, but not from TATA-less promoters, irrespective of whether they were transcribed by Pol II or Pol III (PMID:7510635). This antibody is reported to successfully recognize the polyglutamine chains of mutated alleles of protein from several human neurodegenerative diseases caused by a CAG repeat expansion, like Huntington's disease and spinocerebellar ataxia type 2, 3 and 7 (PMID: 7477379). It is reported that this antibody recognises the linear and extended conformations of polyglutamine (PMID: 23777629).
Antibody First Published
Lescure et al. The N-terminal domain of the human TATA-binding protein plays a role in transcription from TATA-containing RNA polymerase II and III promoters. EMBO Journal (1994); Vol. 13 ;no.5 pp. 1; 166 - 1175. PMID:7510635
Note on publication
Describes the characterization and role of this antibody to inhibit in-vitro transcription from TATA-containing promoter.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
