Cat. No.
MABL-2718
Application
functional assay, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Neisseria meningitidis serogroup A and B
Clone No.
MN14C11.6
From
Recombinant Antibody
Specificity
This antibody recognizes the P1.7 loop of the PorA protein of Neisseria meningitidis. Epitope mapping revealed that this antibody specifically binds a sequence 'ASGQ'. This antibody can bind both A and B serotype of Neisseria meningitidis. The major outer membrane protein PorA of Neisseria meningitidis is the target for bactericidal serosubtyping antibodies and is currently considered as a potential vaccine candidate against group B meningococcal disease.
Alternative Names
PIA; Protein IA; Class 1 protein; P1.7; P1.7 serosubtype; Major outer membrane protein P.IA; serogroup A; serogroup B
UniProt
P57041; E6MXW0
Immunogen
The original antibody was generated immunizing mice with outer membrane protein preparations from Neisseria meningitidis.
Application Notes
This antibody showed both bactericidal and opsonophagocytic for P1.7-expressing meningococci. The lowest concentration resulting in >50% killing of strain 44/76-SL was reported to be 0.5 µg/l. The opsonic activity of this antibody was measured against strain 44/76-SL with human peripheral blood polymorphonuclear leukocytes (PMN) as the effector cells and human serum as the complement source. This antibody demonstrated high opsonophagocytic activity with >80% of PMNs exhibiting measurable RB at a concentration of the MAbs of 10 μg/ml. This antibody was opsonic for the test strain 44/76-SL at a concentration of 0.2 μg/ml (PMID: 10722576). This antibody is not capable of binding meningococcal isolates expressing some variations of the P1.7 family especially P1.7b in a whole cell ELISA (PMID: 10512717). This antibody can be used in the detection of outer membrane protein of N. meningitidis strains like 44/76 using an ELISA (PMID: 12753502). This antibody was also used for the analysis of isogenic target strains derived from H44/76 (B:15:P1.7,16) in a western blot assay (PMID: 8698504).
Antibody First Published
Abdillahi et al. Definition of meningococcal class 1 OMP subtyping antigens by monoclonal antibodies. FEMS Microbiol Immunol. 1988 Dec;1(3):139-44. PMID:3152545
Note on publication
The paper defines antigenic determinants on the class 1 outer membrane proteins
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
