Cat. No.
MABL-2725
Application
crystallisation, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
13G10
From
Recombinant Antibody
Specificity
13G10 was shown to bind Fe(ToCPP) using competitive ELISA with an affinity of 20 nM. 13G10 showed that same affinity for non-metallated Fe(ToCPP). The antibody did not bind meso- tetraphenylporphyrin, suggesting that the carboxylate groups are critical for recognition.
Alternative Names
Fe(ToCPP); Iron(III)-meso-alpha,alpha,alpha,beta-tetrakis- orthocarboxyphenylporphyrin
UniProt
Immunogen
BALB/c mice were immunised by injection of iron(III)-meso-alpha,alpha,alpha,beta-tetrakis- orthocarboxyphenylporphyrin (Fe(ToCPP)) conjugated to KLH. Splenocytes were obtained from immunised mice and fused with the PA1 myeloma cell line to generate a hybridoma.
Application Notes
13G10 has been used in ELISAs to show binding to various porphyrin molecules, with different metallation states and functional groups (Quilez et al, 1996). The antibody was also shown to exhibit catalytic activity when incubated with Fe(ToCPP) and ABTS and hydrogen peroxide, however was later shown to also use a wider range of hydroperoxides in catalysis (de Lauzon et al, 1999).
Antibody First Published
Quilez et al. Artificial peroxidase-like hemoproteins based on antibodies constructed from a specifically designed ortho-carboxy substituted tetraarylporphyrin hapten and exhibiting a high affinity for iron-porphyrins. FEBS Lett. 1996 Oct 14;395(1):73-6. PMID:8849692
Note on publication
Describes the generation of 3 mAbs against a modified porphyrin derivative (Fe(ToCPP)) and characterisation of their binding to porphyrin molecules, as well as catalytic peroxidase activity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
