Cat. No.
MABL-2729
Application
functional assay, SPR, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
10C12
From
Recombinant Antibody
Specificity
This antibody is specific for oxidized phospholipids POVPC and PGPC. These are lipids play an important role in cardiovascular disease.
Alternative Names
POVPC; PGPC
UniProt
Immunogen
The mouse version of this antibody was generated by feeding an ApoE KO mice with a high- fat western diet.
Application Notes
When injected in ApoE KO mice, the mouse version of this antibody decreased accumulation of macrophages in the brachiocephalic artery and decreased OxPL-induced inflammatory response (Cherepanova and Owens, 2014). To study the binding of its ligands a surface plasmon resonance experiment was preformed on POVPC, PGPC and DMPC using the mouse version of this antibody. Modified Russell-Movat (Movat) staining for morphometric analysis of the brachiocephalic arteries was preformed using the mouse version of this antibody. To confirm the binding of this antibody to POVPC and PGPC an ELISA was preformed (Cherepanova et al, 2019; pmid:31645128).
Antibody First Published
Cherepanova et al. Novel Autoimmune IgM Antibody Attenuates Atherosclerosis in IgM Deficient Low-Fat Diet-Fed, but Not Western Diet-Fed Apoe-/- Mice Arterioscler Thromb Vasc Biol. 2020 Jan;40(1):206-219. PMID:31645128
Note on publication
A novel IgM autoantibody was generated, 10C12, from the spleens of Apoe-/- mice fed a long-term Western diet, that demonstrated potent OxPL neutralizing activity in vitro and the ability to inhibit macrophage accumulation within arteries of Apoe-/- mice fed a Western diet for 4 weeks.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
