Cat. No.
MABL-2730
Application
IP, WB, IF
Isotype
Engineer antibody
Species Reactivity
Birds, Mink, Rat, Human, Mouse
Clone No.
GD11
From
Recombinant Antibody
Specificity
This antibody is specific for pp60src. It recognizes this protein in avians, humans and different rodents. It recognizes amino acids 82-169. Pp60src is a non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors.
Alternative Names
Proto-oncogene tyrosine-protein kinase Src; SRC; Proto-oncogene c-Src; pp60c-src; p60-Src
UniProt
P00523
Immunogen
The mouse version of this antibody was generated by immunizing a mouse with recombinant chicken pp60src.
Application Notes
To confirm wether a 60,000-Mr phosphoprotein was pp60src, an immunoprecipitation was preformed using the mouse version of this antibody. Furthermore, PrARSV-infected CE cells were immunostained using this antibody (Parsons et al, 1984; pmid:6205164). To determine wether pp60src is present in human T cells, an immunoprecipitation and subsequent western blot were preformed using the mouse version of this antibody on human T cell cultures (Branch and Mills, 1995; pmid:7535811). Proteins from fetal rat lung cells were immunoblotted using the mouse version of this antibody (Liu et al, 1996; pmid:8636139). While researching store-mediated Ca2+ entry, a western blot was preformed on human blood cells using the mouse version of this antibody (Redondo et al, 2005; pmid:15894173).
Antibody First Published
Parsons et al. Monoclonal antibodies to Rous sarcoma virus pp60src react with enzymatically active cellular pp60src of avian and mammalian origin J Virol. 1984 Aug;51(2):272-82. PMID:6205164
Note on publication
The derivation and characterization of 22 hybridoma clones producing monoclonal antibodies (Mabs) specific for the transforming protein of Rous sarcoma virus, pp60src, are described.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
