Cat. No.
MABL-2751
Application
Block, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
DB3
From
Recombinant Antibody
Specificity
The antibody binds progesterone with an affinity (Kd) of 1 nM.
Alternative Names
P4; D
UniProt
Immunogen
11-a-hydroxyprogesterone-11-hemisuccinyl-bovine serum albumin (BSA) conjugate.
Application Notes
The antibody binds specifically to progesterone, a steroid female sex hormone produced by the corpus luteum in the ovaries, and then by the placenta during pregnancy. The hormone is necessary for maintenance of pregnancy as it prevents the breakdown of the endometrium. Monoclonal antibodies against progesterone can be useful to increase the understanding of the role of the hormone in the female reproductive system, and may be potentially used as an immunological prevention of pregnancy. Studies have shown that the injection of DB3 in female mice had anti-fertility effects. It is thought that DB3 may inhibit pregnancy by binding to a progesterone transport protein, thereby preventing the uptake of progesterone by epithelial cells of the uterine tissue.
Antibody First Published
Stura EA, Arevalo JH, Feinstein A, Heap RB, Taussig MJ, Wilson IA. Analysis of an anti-progesterone antibody: variable crystal morphology of the Fab' and steroid-Fab' complexes. Immunology. 1987 Dec;62(4):511-21. PMID:3123368
Note on publication
Describes the production of an anti-progesterone monoclonal antibody DB3, which can be used to terminate pregnancy shortly after fertilization, and for hormone immunoassay.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
