Cat. No.
MABL-2772
Application
ICC, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MIL81-1-8
From
Recombinant Antibody
Specificity
This antibody is specific for p-Y525-SYK.
Alternative Names
Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk
UniProt
P43405
Immunogen
The original antibody was raised by immunizing New Zealand White rabbits with a peptide covering residues 520-529 of hSYK and residues corresponding to tyrosine 525 and tyrosine 526 are phosphorylated.
Application Notes
ELISA was performed on GST-SYK-p using this antibody (WO2015164330). Western blot was performed with this antibody on GST-pSYK (IVK), and phosphatase- treated GST-pSYK (WO2015164330). AlphaLISA® luminescence proximity assay was performed on IVK and PPase treated recombinant GST-SYK samples using this antibody (WO2015164330). This antibody was used for immunofluorescence on HeLa cells transfected for 24 hr with lipid: DNA complex (WO2015164330).Immunocytochemistry was performed using this antibody on WSU-DLCL2 samples untreated or activated by Fab crosslinking (WO2015164330). Immunohistochemistry was performed on PHTX-95L (WO2015164330). This antibody was also used for immunohistochemistry on BLl xenograft tissue and several primary human tissue xenografts (PHTX-95L, OCI LY10) (WO2015164330).
Antibody First Published
Brake et al. Anti-psyk antibody molecules and use of same for syk-targeted therapy. WO2015164330
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
