Cat. No.
MABL-2775
Application
functional assays, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
3RH11
From
Recombinant Antibody
Specificity
This antibody recognizes puromycin, an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation. Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis.
Alternative Names
aminonucleoside antibiotic puromycin
UniProt
Immunogen
The original mouse IgG1 antibody was raised against puromycin hydrochloride.
Application Notes
This monoclonal antibody to puromycin provides a non-radioactive method to measure rates of global protein synthesis (mRNA translation) in cells or tissue slices incubated with puromycin, or animals treated with puromycin in vivo (Frolinger et al., 2018; pmid: 29702026). 3RH11 offers advantageous alternative to traditional pulse-chase methods, which rely on radioactive amino acid labeling as it allows for the simple evaluation and quantification of translation directly using standard immunochemical methods (Balukoff et al., 2020; pmid: 33188200). Classical pulse-chase or flooding dose methods used to monitor protein synthesis rely on the measurement of radioactive methionine and cysteine labels. Analysis using puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling, and allows for the evaluation/quantification of translation directly using standard immunochemical methods (Frolinger et al., 2018; pmid: 29702026).
Antibody First Published
Kelleher et al. The mTORC1 signaling repressors REDD1/2 are rapidly induced and activation of p70S6K1 by leucine is defective in skeletal muscle of an immobilized rat hindlimb Am J Physiol Endocrinol Metab. 2013 Jan 15;304(2):E229-36. doi: 10.1152/ajpendo.00409.2012. Epub 2012 Nov 27. PMID:23193052
Note on publication
Describes the method of measuring protein synthesis using an anti-puromycin antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
