Cat. No.
MABL-2776
Application
in vitro neutralization, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Rabies Virus
Clone No.
III20
From
Recombinant Antibody
Specificity
The antibody binds the PV11 strain of the rabies virus.
Alternative Names
UniProt
Immunogen
The original scFv antibody was selected from a human synthetic scFv phage display library by panning against the PV11 strain of the virus.
Application Notes
The specificity of the scFv-Fc format of the antibody was confirmed by ELISA analysis. The scFv-Fc version of the antibody detected GPRV by western blot analysis. The scFv-Fc version of the antibody bound specifically to the membranes of PV11 infected Vero cells in an immunofluorescence assay. The scFv-Fc version of the antibody neutralized the PV11 strain in a standard in vivo neutralization assay where the virus was incubated with the scFv-Fc fragment before intracranial inoculation in mice (Ray et al., 2001; PMID: 11472431).
Antibody First Published
Ray et al. Selection of single chain variable fragments (scFv) against the glycoprotein antigen of the rabies virus from a human synthetic scFv phage display library and their fusion with the Fc region of human IgG1 Clin Exp Immunol. 2001 Jul; 125(1): 94–101. PMID:11472431
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
