Cat. No.
MABL-2780
Application
ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
1H6
From
Recombinant Antibody
Specificity
The antibody binds to quadruplex DNA with a Kd of 0.33 nM. Binds to both types of quadruplex DNA, Oxy-2 (d(TTTTGGGG)2) and Ver-3 (d(TGGGGG(TTAGGG)2T).
Alternative Names
G4 DNA
UniProt
Immunogen
Oxy-2 or d(TTTTGGGG)2.
Application Notes
This antibody binds to quadruplex DNA, which is generated through the association of four guanines bound through Hoogsteen base pairing. G4-forming sequences are associated with immunoglobulin switch regions, promoter sequences, rDNA and telomeric repeats. Ab00389-1.1 was used by Hansel-Hertsch et al. 2016 (PMID: 27618450) in immunofluorescence staining (in comparison to the BG4 anti-G quadruplex antibody, c.f. Ab00174).
Antibody First Published
Henderson et al. Detection of G-quadruplex DNA in mammalian cells. Nucleid Acids 2014; 42(2):860-869 PMID:24163102
Note on publication
Describes the generation of several G4-DNA-specific monoclonal antibodies and how these can be used for nuclear staining.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
