Cat. No.
MABL-2779
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
3C8
From
Recombinant Antibody
Specificity
This antibody reacts specifically with pyrrolated protein and does not cross react with DNA. Lysine N-pyrrolation is a post translational modification, a unique covalent modification that converts lysine residues to Nε-pyrrole-L-lysine (pyrK). It imparts electronegative properties to proteins, causing them to mimic DNA and the proteins acquire an electrical conductivity via lysine N-pyrrolation.
Alternative Names
pyrP; Lysine N-pyrrolation; pyrK
UniProt
Immunogen
The original antibody was isolated from the repertoire of the B-1a cell populations in the apoE deficient (apoE−/−) mice. Spleen cells from male apoE−/− mice at 21 weeks of age were fused with P3/U1 murine myeloma cells, and after screening based on specific binding to pyrBSA
Application Notes
This antibody is recommended for determination of pyrrolated proteins in a sample. The binding reactivity of this antibody towards pyrrolated-BSA protein was determined using ELISA. This antibody does no cross react with DNA in ELISA (PMID: 35031322).
Antibody First Published
Lim et al. Unique B-1 cells specific for both N-pyrrolated proteins and DNA evolve with apolipoprotein E deficiency. J Biol Chem. 2022 Jan 11;101582. PMID:35031322
Note on publication
This paper evaluates the role of pyrrolated proteins in innate immunity using antibodies from apoE-deficient (apoE−/−) hyperlipidemic mice.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
