Cat. No.
MABL-2796
Application
SPR, Western blot, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2A11
From
Recombinant Antibody
Specificity
2A11 is specific for the V-domain of human advanced glycosylation end product-specific receptor. It recognises both the glycosylated and non-glycosylated forms.
Alternative Names
AGER; advanced glycosylation end product-specific receptor; receptor for advanced glycosylation end products
UniProt
Q15109
Immunogen
Human RAGE protein.
Application Notes
ELISA confirmed the specificity of 2A11 for the V-domain of RAGE, with a binding affinity (Kd) of 7.6 nM. ELISA was also used to confirm that the binding of 2A11 to RAGE is glycosylation independent. In SPR experiments the Kd of 2A11 binding to RAGE was determined as 0.38 nM. Flow cytometry and immunochemistry were used to visualise the binding of 2A11 to HEK-RAGE cells.
Antibody First Published
Jyoti, 2012. Development of new antibody based theranostic agents targeting the receptor for advanced glycation end-product (RAGE). Dissertation Submitted to the Graduate Faculty of the North Dakota State University of Agriculture and Applied Science
Note on publication
Describes the characterisation of 2A11 through ELISA, SPR, FC, and IH experiments.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
