Cat. No.
MABL-2804
Application
neutralising, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
IK22-5
From
Recombinant Antibody
Specificity
This antibody is specific for murine RANKL.
Alternative Names
CD254; IK22/5; RANK Ligand; Tumor necrosis factor ligand superfamily member 11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; TNF-related activation-induced cytokine; TRANCE
UniProt
O35235
Immunogen
Thus antibody was raised by immunising an SD rat with murine RANKL-transfected baby hamster kidney cells.
Application Notes
This antibody has been used in immunohistochemical analysis of frozen Peyer's patch tissue sections (Kobayashi et al, 2013). It has also been used in flow cytometric analysis of RANKL cell surface expression by Th1 and Th17 cells (Nakae et al, 2007), RANKL-transfected human embryonic kidney cells (Miyahira et al, 2003) and murine T hybridoma 2B4.11 cells (Fionda et al, 2007). Additionally, this RANKL-neutralising antibody has been used to assess the effects of RANKL inhibition on myoepithelial cell proliferation in mammary organoid culture (Haslam et al, 2008). Treatment of collagen-induced arthritis (CIA) mice with this antibody has been shown to reduce bone loss at the site of inflammation (Kamijo et al, 2006), while administration to C57BL/6J mice induced transient tibia and skull bone growth arrest and irreversible blockage of tooth eruption (Lezot et al, 2015).
Antibody First Published
Kamijo et al. Amelioration of bone loss in collagen-induced arthritis by neutralizing anti-RANKL monoclonal antibody. Biochem Biophys Res Commun. 2006 Aug 18;347(1):124-32. PMID14:21:16815304
Note on publication
Describes the original generation of this antibody, and the characterisation of its effects when administered to collagen-induced arthritis mice.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
