Cat. No.
MABL-2805
Application
IP, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
7F1
From
Recombinant Antibody
Specificity
This antibody is specific for human RAP. It does not cross-react with rabbit or murine RAP.
Alternative Names
Receptor-associated protein; low density lipoprotein receptor-associated protein; 39-kDa receptor-associated protein
UniProt
Immunogen
This antibody was raised by immunising mice with human placental RAP.
Application Notes
This antibody has been used to assess human RAP expression by RAP-transfected H4 cells, derived from human neuroglioma cells, in immunostaining analysis (Kinoshita et al, 2001). It has also been used in immunohistochemical analysis of paraffin-embedded human kidney tissue sections (Kounnas et al, 1992), and in ELISA analysis to detect RAP binding (Medh et al, 1995; Kounnas et al, 1993). dditionally, this antibody has been used to immunoprecipitate RAP, and in western blot analysis of purified B-amyloid protein and RAP mixtures (Kerr et al, 2010).
Antibody First Published
Kounnas et al. The 39-kDa receptor-associated protein interacts with two members of the low density lipoprotein receptor family, alpha 2-macroglobulin receptor and glycoprotein 30. J Biol Chem. 1992 Oct 15;267(29):21162-6. PMID:1400426
Note on publication
Describes the original generation of this antibody, and its use in immunohistochemistry.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
