Cat. No.
MABL-2806
Application
IF/ICC, IP, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
4C8/1
From
Recombinant Antibody
Specificity
This antibody recognises human and mouse RAP1, which is a part of the shelterin complex involved in the regulation of telomere length and protection. Additionally, RAP1 acts as transcription factor at extratelomeric sites regulating gene expression.
Alternative Names
TERF2IP; Telomeric repeat-binding factor 2-interacting protein 1; TERF2- interacting telomeric protein 1; TRF2-interacting telomeric protein 1; Dopamine receptor-interacting protein 5; Repressor/activator protein 1 homolog; RAP1 homolog; hRap1; 4C8; 4C8 1
UniProt
Q9NYB0
Immunogen
This antibody was raised against human RAP1.
Application Notes
WB and IHC: RAP1 was visualised multiple times in different Western blot analyses using RAP1 4C8/1 antibody; furthermore, this antibody was used to identify RAP1 immunohistochemically in invasive ductal carcinoma samples in experiments on the RAP1's role in regulating NF-kappaB (Teo et al., 2010). WB: Tascher et al. (2017) compared RAP1 protein amounts in mice skeletal muscle samples on Western blots using this antibody to investigate changes in expression of telomeric proteins during space flights. IF/ICC: RAP1 was successfully visualised in hMSCs using RAP1 4C8/1 antibody (Zhang et al., 2019).
Antibody First Published
Bombarde et al. TRF2/RAP1 and DNA-PK mediate a double protection against joining at telomeric ends. EMBO J. 2010 May 5;29(9):1573-84. doi: 10.1038/emboj.2010.49. Epub 2010 Apr 20. PMID:20407424
Note on publication
This article describes the earliest published use of anti-RAP1 4C8/1 antibody in Western blot analyses in the experiments on the mechanisms preventing end-joining in telomeric regions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
