Cat. No.
MABL-2833
Application
functional assays
Isotype
Engineer antibody
Species Reactivity
Trypanosoma cruzi
Clone No.
B10
From
Recombinant Antibody
Specificity
This antibody recognises DDxGF sequence of the C-terminal of eukaryotic ribosomal P proteins.
Alternative Names
60S acidic ribosomal protein P2-B; P2B; R13; P2b; TcP2b; R13
UniProt
P26795
Immunogen
This antibody was derived from BALB/c mice immunised with TcP2b.
Application Notes
Both B10 mAb and scFv were demonstrated to detect eukaryote ribosomal P proteins with a high specificity demonstrated by the alanine-scanning mutagenesis of peptide R13 (López Bergami et al., 2003). Consequently, this antibody can be used in the detection of ribosomal P proteins in various eukarote tissues. Finally, anti-P protein antibodies are observed in certain pathologies, such as Trypanosoma cruzi infection and SLE, and thus B10 antibody might be utilised as a model for the investigation of the immunology of these diseases (López Bergami et al., 2003).
Antibody First Published
López Bergami et al. Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins. Biochem Biophys Res Commun. 2003 Feb 21;301(4):819-24. PMID:12589786
Note on publication
This article describes the generation and characterisation of the B10 monoclonal antibody as well as its scFV derivative.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
