Cat. No.
MABL-2836
Application
crystallography, neutralizing, ELISA
Isotype
Engineer antibody
Species Reactivity
Ricinus communis
Clone No.
F8(JOB10)
From
Recombinant Antibody
Specificity
The antibody is specific for RTA. The antibody's principal contacts with RTA were centered on β–strand h, with lesser interactions occurring with α–helix B and α–helix D. Ricin is a member of the type II ribosome-inactivating protein (RIP) family of plant toxins. It is a 65 kDa glycoprotein consisting of two subunits, RTA and RTB, joined by a single disulfide bond.
Alternative Names
rRNA N-glycosidase; Ricin A chain (EC:3.2.2.22); Ricin B chain
UniProt
P02879
Immunogen
The original antibody was generated by immunizing alpacas, followed by library construction and panning on RTA.
Application Notes
The crystal structure of the VHH antibody in complex with the ricin toxin's enzymatic subunit (RTA) was solved. The specificity of the original format of the antibody was confirmed by ELISA analysis, and the antibody's EC50 for ricin holotoxin was 0.5-0.8 nM. The binding affinity of the VHH fragments to ricin was measured by surface plasmon resonance (Kd= 0.2 nM). In the Vero cell cytotoxicity assay, the VHH had an IC50 of ∼300 nM, thereby classifying it as a weak neutralizing antibody (Rudolph et al., 2016; PMID: 27159829).
Antibody First Published
Rudolph et al. Structural analysis of nested neutralizing and non-neutralizing B cell epitopes on ricin toxin's enzymatic subunit. Proteins. 2016 Aug;84(8):1162-72. PMID:27159829
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
