Cat. No.
MABL-2839
Application
crystallization
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
BL3-6S97N
From
Recombinant Antibody
Specificity
The antibody binds to the RNA hairpins GAAACAC hairpin. An RNA hairpin is an essential secondary structure of RNA. It can guide RNA folding, determine interactions in a ribozyme, protect messenger RNA (mRNA) from degradation, serve as a recognition motif for RNA binding proteins or act as a substrate for enzymatic reactions.
Alternative Names
UniProt
Immunogen
A library of Fabs was generated by rationally varying the composition of the residues in complementarity determining regions (CDRs) of Fab BL3–6. Subsequently, the Fab library was challenged in a phage display selection to recognize and bind the GAAACAC motif.
Application Notes
The Fab was generated by directed mutagenesis from the parental Fab BL3–6. The S97N mutation in the complementarity determining region L3 of the Fab improved the binding affinity compared to the parent Fab BL3–6 (Kd = 5 nM). The Fab fragment was also found to bind the affinity- matured GAGACCC hairpin. The crystal structure of the Spinach RNA grafted with GAAACAC loop in complex with Fab BL3–6S97N was determined (Koirala et al., 2018; PMID: 29309709).
Antibody First Published
Koirala et al. Affinity maturation of a portable Fab–RNA module for chaperone-assisted RNA crystallography Nucleic Acids Res. 2018 Mar 16;46(5):2624-2635. PMID:29309709
Note on publication
The original paper describes the use of Fab fragment as chaperone for RNA crystallography.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
