Cat. No.
MABL-2838
Application
Blocking, functional assay, IP, SPR, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MB2A4
From
Recombinant Antibody
Specificity
This antibody is an idiotypic antibody against rituximab. It shows no cross-reactivity with other CD20-specific antibodies. Rituximab is a monoclonal antibody against CD20 used to treat autoimmune diseases and some types of cancer.
Alternative Names
Rituximab; Rituxan
UniProt
Immunogen
The rat version of this antibody was raised by immunizing a rat with the F(ab)2 fragment of Rituximab.
Application Notes
To develop an ELISA to measure rituximab, an ELISA was performed on solutions of rituximab using the rat version of this antibody. The ELISA could be used to quantify rituximab at concentrations between 0.125 and 50 μg/mL. Furthermore, the binding strength of this antibody to rituximab was measured using SPR. It showed a Kd value of 0.6 nM. Finally, a competition assay between this antibody and a P20 peptide was performed to determine whether P20 could block the binding of this antibody. A decrease in binding was indeed noted (Blasco et al., 2007; PMID: 17651747). To characterize this antibody, an ELISA was performed on diluted samples of rituximab using the rat version of this antibody. Furthermore, flow cytometry was used to detect rituximab on the surface of CD20-positive cells using this antibody. The antibody was bound to rituximab-coated cells and showed no cross-reactivity with other CD20-binding antibodies. The ability of this antibody to block rituximab from binding B cells was also shown (Cragg et al., 2004; PMID: 15213098).
Antibody First Published
Cragg et al. A new anti-idiotype antibody capable of binding rituximab on the surface of lymphoma cells Blood. 2004 Oct 15;104(8):2540-2. PMID:15213098
Note on publication
An anti-idiotype antibody against rituximab was raised and characterized.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
