Cat. No.
MABL-2841
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
calf
Clone No.
cAb-RN05
From
Recombinant Antibody
Specificity
This antibody binds bovine RNase A. RNase A is an endonuclease enzyme that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. It acts on single-stranded and double-stranded RNA.
Alternative Names
Ribonuclease pancreatic; RNASE1; RNase 1; RNaseA; RNS1 RN05
UniProt
P61823
Immunogen
The original antibody was generated by immunizing a dromedary (Camelus dromedarius) with bovine RNase A. The affinity matured version of this antibody was generated using synthetic combinatorial libraries.
Application Notes
This antibody binds bovine RNase with an affinity in the picomolar range. This affinity matured version of VHH antibody cAb-RN05 binds bovine RNase with an affinity of Kd= 180 pM (PMID: 17888451). 5′–3′ cUMP that binds into the active site of RNase A does not compete with cAb-RN05 for binding to RNase A in an ELISA (PMID: 10196124).
Antibody First Published
Koide et al. Exploring the capacity of minimalist protein interfaces: interface energetics and affinity maturation to picomolar KD of a single-domain antibody with a flat paratope. J Mol Biol. 2007 Nov 2;373(4):941-53. PMID:17888451
Note on publication
Describes the affinity maturation of a VVH antibody against bovine RNase.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
