Cat. No.
MABL-2842
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
R2E5
From
Recombinant Antibody
Specificity
The antibody is specific for Ro52. Ro52 is a protein of 52 kDa and it is pard of Ro RNP. Ro52 is an autoantigen associated with SS and systemic lupus erythematosus (SLE).
Alternative Names
Ro ribonucleoprotein particle; Ro RNPs
UniProt
Immunogen
The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens α-fodrin, hPop1, HspB3, Ro52, Rpp30, Rrp4 and Rrp42 in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis (Hof et al., 2008; PMID: 18062792).
Antibody First Published
Hof et al. Multiple-antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens Clin Exp Immunol. 2008 Feb;151(2):367-77. PMID:18062792
Note on publication
Describes the generation of chicked antibodies against various human autoantigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
