Cat. No.
MABL-2851
Application
FACS, in vivo, SPR
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
89M5
From
Recombinant Antibody
Specificity
The antibody specifically binds human RSPO1. The antibody cross-reacts with mouse RSPO1
Alternative Names
R-spondin-1; Roof plate-specific spondin-1; hRspo1
UniProt
Q2MKA7
Immunogen
The original antibody was generated by immunizing mice against recombinant human RSPO1 protein amino acids 31-263.
Application Notes
The binding affinity of the original format of the antibody to human and mouse RSPO1 was measured by surface plasmon resonance (Kd less than 0.1 nM respectively). Further, the binding affinity of the humanized version of the antibody, h89M5-H2L2, to human RSPO1 was measured by surface plasmon resonance (Kd less than 0.1 nM). The original format of the antibody could inhibit tumor cell growth in vivo in an ovarian tumor xenograft model as single agents and in combination with a chemotherapeutic agent. The original format of the antibody could inhibit tumor cell growth in vivo in a pancreatic tumor xenograft model in combination with a chemotherapeutic agent. The original antibody was able to block RSPO1-induced β-catenin signaling in HEK-293 cells. The original antibody blocked binding of RSPO1 to LGR5 in HEK-293 cells, as measured by FACS. The original and humanized format of the antibody, could bind to human RSPO1 expressed on HEK-293 cells, as shown by FACS analysis (US8802097B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
