Cat. No.
MABL-2852
Application
Blocking, in vitro, in vivo, SPR
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
h130M23-H1L2
From
Recombinant Antibody
Specificity
The antibody specifically reacts with human and mouse RSPO2.
Alternative Names
hRspo2; R-spondin-2; Roof plate-specific spondin-2
UniProt
Q6UXX9
Immunogen
The antibody is the humanized version of the murine 130M23.
Application Notes
The antibody was shown to completely block β-catenin signaling, demonstrating it is a specific inhibitor of RSPO2. The antibody blocked soluble RSPO2 binding to LGR5. The antibody had a binding affinity for human RSPO2 of 0.14 nM and mouse RSPO2 of 0.35 nM. The antibody demonstrated strong anti-tumor growth activity in combination with gemcitabine in a pancreatic xenograft model and inhibition of pancreatic tumor growth in vivo as a single agent and in combination with additional therapeutic agents. The humanized version of the antibody was produced. The humanized version had a binding affinity for human RSPO2 of 0.13 nM (US8802097B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
