Cat. No.
MABL-2853
Application
NTRL, therapeutic, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
RSV
Clone No.
MEDI-493 (Palivizumab, hu129)
From
Recombinant Antibody
Specificity
This antibody recognizes the site A of the RSV F glycoprotein. This antibody binds the 'NSELLSLINDMPITNDQKKLMSNN' epitope (PMID: 20098425).
Alternative Names
F2; Fusion glycoprotein F0; Protein F; F gycoprotein; Human respiratory syncytial virus; humanized mAb 129
UniProt
P03420
Immunogen
The original antibody was generated by immunizing female BALB/c mice by primary intranasal infection with A2 strain of RSV.
Application Notes
This broadly neutralizing antibody is recommended to be used as a prophylactic agent for infants with risk for RSV infections. The binding and functional properties of this antibody were studied using ELISA and in vitro neutralization assays. The affinity of MEDI-493 was found to be 1.4 nM, equal to or slightly better than an isotype matched chimeric derivative of mAb 1129 (Johnson et al., 1997).Pretreatment of cotton rats with MEDI-493 resulted in 99% reduction of lung RSV titers at a dose of 2.5 mg/kg, corresponding to a serum concentration of 25-30 microg/mL (PMID: 9359721). MEDI-493 was safe and well-tolerated in this high risk pediatric population (PMID: 9493805). It was reported that monthly intramuscular doses of 15 mg/kg maintained mean trough serum concentrations that were above 40 microg/ml (the value associated with 99% reduction of pulmonary RSV in the cotton rat model) (PMID: 9779762).
Antibody First Published
Johnson et al. Development of a humanized monoclonal antibody (MEDI-493) with potent in vitro and in vivo activity against respiratory syncytial virus. J Infect Dis. (1997); 176(5):1215-24. PMID:9359721
Note on publication
Describes the generation of humanized version of mAb 1129 against a conserved neutralizing epitope on the F glycoprotein of RSV.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
