Cat. No.
MABL-2854
Application
Depletion, IP, WB, FC, IF
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-17
From
Recombinant Antibody
Specificity
The OX-17 antigenic determinant is isotypic and on the alpha chain. OX-17 labels B but not T cells from lymph node or thoracic duct lymph but very weakly labels thymocytes and does not label erythrocytes. OX-17 does not cross-react with Rat Ia-A or Mouse Ia-E antigen. The target antigen is expressed on antigen presenting cells, including B cells, macrophages and dendritic cells. RT1-D is a rat MHC class II protein. RT1-D is equivalent to mouse I-E antigen.
Alternative Names
MHC class II RT1D alpha chain antigen; OX17; MRC OX-17; MRC OX17
UniProt
O98172
Immunogen
OX-17 was generated using BALB/c mice raised against rat spleen glycoproteins depleted of the Ia-A antigen using an OX-6 immunoaffinity column.
Application Notes
This antibody binds to rat MHC Class I, and has been used to show that, in an in vivo rat model, antibodies against MHC class I antigens from stored blood may precipitate transfusion-related acute lung injury (Kelher, 2009).
Antibody First Published
Fukumoto et al Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol 12:237-43 19826456222 PMID:6178598
Note on publication
Describes the generation and use of OX-17 to identify and purify novel Ia antigens..
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
